What is a Testicular Tissue Biopsy?
A testicular biopsy is a procedure in which a small tissue sample is removed from one or both testicles for laboratory analysis.
It is used to:
- Diagnose the causes of male infertility, particularly when sperm production is abnormal or absent (azoospermia)
- Retrieve sperm for in vitro fertilisation (IVF) when sperm are present in the testicles but not in the semen
- Diagnose testicular cancer or other testicular abnormalities
The biopsy can be performed as a percutaneous (needle) procedure or an open (surgical) procedure. In both cases, a small sample of testicular tissue is extracted for examination.
Testicular biopsy can help determine if the issue is due to a blockage or problems with sperm production within the testicles.
When is a Testicular Tissue Biopsy Recommended?
Testicular biopsy is often recommended when semen analysis shows a complete lack of sperm. It can help determine if the issue is due to a blockage (obstructive azoospermia) or problems with sperm production within the testicles (non-obstructive azoospermia).
Additionally, a testicular biopsy is performed to retrieve sperm directly from the testicles when it is not present in the semen. The extracted sperm can then be used for IVF procedures.
Testicular biopsy may also be recommended when the cause of male infertility is unclear, and after other tests like semen analysis and hormone testing have not provided a definitive diagnosis. The biopsy can help identify underlying conditions like testicular cancer or other abnormalities that may be impacting fertility.
Testicular Tissue Biopsy: How is it Done?
The biopsy can be performed in two main ways:
Open (Surgical) Biopsy:
- The doctor makes a small incision in the scrotum to expose the testicle.
- A small sample of testicular tissue is then removed using surgical instruments.
- The incision is closed with stitches.
Percutaneous (Needle) Biopsy:
- The doctor uses a thin needle to extract a sample of testicular tissue through the skin of the scrotum.
- This can be done using a fine needle or a core needle biopsy, which takes a larger tissue sample.
- No incision is made, and the needle puncture site is simply covered with a bandage.
Why Choose Us
Choosing the right fertility clinic is crucial for starting your family. At Birla Fertility & IVF, we offer personalised care with expert specialists guiding you every step of the way. Our advanced labs and outstanding success rates have helped over 2,30,000 patients achieve their dream of parenthood.

Azoospermia
Treatment depends on whether sperm production or blockage is the cause.
Advanced techniques can help retrieve sperm in many cases.

Bartholin Cysts Treatment
Treated with the help of medication or minor procedures to relieve pain and prevents infection.

Blocked Fallopian Tube
Mostly treated with surgery or IVF, depending on the severity of the blockage to prevent delays in conception.

Cervical Cancer
Cervical cancer depends on the stage and may include surgery or therapy. Regular screening helps detect it early and improve outcomes.
Chocolate Cysts
Managed with medication or minimally invasive surgery based on the symptoms and the size of the chocolate cysts.

Ectopic Pregnancy Treatment
Either surgery (laparoscopy/laparotomy) to remove the tissue or medication (methotrexate) to limit growth, selected based on early diagnosis, hormone levels, and stability, with the goal of preventing life-threatening rupture.

Egg Freezing
A woman’s eggs are retrieved, frozen, and stored for future use, allowing her to delay pregnancy for personal, medical, or professional reasons.

Erectile Dysfunction
Managed with medication, lifestyle changes, or therapy. Treating the root cause restores confidence and sexual health.

Hormonal Imbalance
Treated using hormonal therapy and lifestyle changes which helps balance improves cycles and ovulation.
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