The sample is frozen using liquid nitrogen which is at a temperature of -196°C. Successful cryopreservation involves draining cell water and replacing it with cryoprotectant or antifreeze agents. This is done through simple osmosis. Once frozen, the sperm cells are in suspended animation where all metabolic activity is effectively stopped, enabling it to be stored as long as this temperature is maintained.
How is sperm frozen?
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Dr. Prachi Benara
MBBS (Gold Medalist), MS (OBG), DNB (OBG) PG Diploma in Reproductive and Sexual health
16+ Years of experience