Laser Assisted Hatching (LAH)
Laser-assisted hatching (LAH) is a supplementary procedure for patients undergoing in vitro fertilisation (IVF) treatment. It helps the embryo hatch from its shell and improves its chances of implanting in the uterus. Those with a history of failed IVF treatments may find this technique helpful.

What is LAH?

In its initial stages, the embryo has a hard outer covering or shell called zona pellucida. After a few days of fertilisation, the embryo hatches out of this shell to implant into the uterus. This results in a pregnancy. However, sometimes, a thicker shell makes it difficult for the embryo to break out.
With LAH, a hole is made in the shell to assist the embryo in hatching out and attaching itself to the uterine wall.
LAH is usually advised by doctors for women above 35 or those who have had failed IVF treatments in the past. It is also done on frozen embryos as freezing can harden the shell.
Fertility treatment providers offer LAH as a complementary procedure to IVF. This artificial support to the embryo improves the chances of implantation rates and a successful pregnancy.
When is LAH recommended?
LAH is typically recommended in the following scenarios:
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Advanced maternal age: In women over 35 with infertility issues, there is a higher likelihood of the zona pellucida failing to break. This is due to the diminished quality of the egg. It further reduces the chances of the embryo implanting itself into the uterus in the IVF treatment. LAH helps break the shell and assists the embryo in attaching to the uterine walls.
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Diminished Ovarian Reserve: This can occur due to injury, disease or as a result of ageing. 10% to 30% of patients with infertility have a diminished ovarian reserve (DOR). IVF and LAH are recommended for women with DOR to increase their chances of pregnancy.
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Repeated IVF Failures: Failed IVF is when good-quality embryos fail to implant or when a pregnancy ends in a miscarriage. IVF failures happen due to chromosomal abnormalities, poor quality of the egg and embryo, uterine abnormalities, high maternal age, sperm quality and the couple’s health condition.

What are the steps of LAH?

The LAH procedure consists of the following steps:
1. Stimulation: First, hormonal stimulation is employed to help produce multiple eggs. These eggs are observed and then retrieved once they reach their ideal size. This step is important as it ensures the availability of enough healthy eggs for the IVF treatment.
2. Fertilisation: These eggs are then exposed to high-quality sperm in a laboratory. This is where fertilisation occurs. These fertilised eggs, also called embryos, are then closely monitored while they grow.
3. Development of the Embryo: An embryologist monitors the development and progress of the embryo until it reaches the blastocyst stage. A blastocyst is made up of two parts: the inner group of cells and the trophectoderm, or the outer group of cells that prompts the uterine contact.
4. Laser Assisted Hatching: This is the stage where a laser is employed to make a crack or a hole in the shell of the embryo. A strong infra-red laser is focused on the zona pellucida under a microscope. It is a quick process of a few seconds that helps the embryo hatch out of the shell.
5. Transferring of the Embryo: Once the embryo hatches out of its shell, it is placed into the mother’s uterus. A thin catheter is used to transfer the embryo with the hope that it implants and initiates a pregnancy.
Why Choose Us
Choosing the right fertility clinic is crucial for starting your family. At Birla Fertility & IVF, we offer personalised care with expert specialists guiding you every step of the way. Our advanced labs and outstanding success rates have helped over 2,30,000 patients achieve their dream of parenthood.
Things to Remember for Laser Assisted Hatching
Before the Treatment
- The LAH procedure is carried out in a laboratory by an embryologist. The embryo is transferred from the culture dish to the dish reserved for the LAH process. The dish is then placed onto a micromanipulation microscope for the laser treatment. The hatched embryo will be delivered into your uterus after the procedure is complete.
During the Treatment
- LAH is carried out three days after the embryo has developed.
- A special laser is used for the procedure that is conducted in a laboratory.
- The laser is focused on the shell of the embryo to make a hole in it.
- It is a safe procedure and the laser does not reach the embryo which ensures its safety.
- The procedure is carried out with extreme care and accuracy and is preferred over chemical or manual methods.
After the Treatment
- While the procedure does not cause any pain, your doctor may give you a mild sedative to help you relax.
- A thin catheter will be placed into your uterus through the vagina.
- A syringe containing the embryo in a fluid will be attached to the other end of the catheter.
- Through the syringe and the catheter, the embryo will be gently placed into your uterus.
- The embryo will attach itself to your uterine lining in about 6 to 10 days.
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