Advanced Semen Analysis

Advanced semen analysis evaluates male fertility by examining sperm parameters and semen characteristics under the supervision of experienced embryologists. The procedure involves the following steps and tests:

1. Sample Collection: Semen is collected through masturbation into a sterile container after 2-5 days of abstinence.

2. Initial Examination: The examiner will assess volume, liquefaction time, and pH level.

3. Sperm Count: Sperm concentration per millilitre using a counting chamber is determined.

4. Motility Assessment: The percentage of sperm with progressive movement is evaluated.

5. Morphology Evaluation: Sperm shape and structure under high magnification according to WHO criteria is analysed.

6. Vitality Testing: Staining techniques will determine the percentage of live sperm.

7. Manual Screening: Experts will manually check for anomalies not detected by automated methods.

8. Computer-Assisted Semen Analysis (CASA): Software is used to analyse sperm motility, concentration, and morphology precisely.

9. DNA Fragmentation Index: Sperm DNA integrity for fragmentation is assessed.

10. Hypo-Osmotic Swelling (HOS) Test: Experts will examine sperm membrane integrity by observing swelling in a hypo-osmotic solution.

11. Antisperm Antibody Test: Antibodies that may bind to sperm and impair function are detected.

Finally, results are interpreted according to established reference values to guide diagnosis and treatment planning.